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KMID : 0380020090240060598
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Korean Journal of Biotechnology and Bioengineering
2009 Volume.24 No. 6 p.598 ~ p.601
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Transformation of Cell Wall-weakened Perilla Seedlings Using Phenolic Compound-treated Agrobacterium Cells and Recombinant Protein Expression
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Chung Il-Kyung
Shin Dong-Il
Park Hee-Sung
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Abstract
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Perilla [Perilla frutescens (L.) Britt] seedlings are easy to grow and eaten as the health vegetable sprout. Two day old perilla seedlings since germination were given a mild wounding using cell wall lytic NaOH/SDS solution for infiltration with recombinant Agrobacterium cells treated with phenolic compounds. In the analysis of fluorometric GUS gene expression for the transformed perilla seedlings, GUS enzyme activity was the highest by the combined treatments of 50 mM acetosyringone and 0.5% NaOH solution containing 0.01% SDS implying a synergic effect. This result could be successfully applied for demonstrating hepatitis B virus antigen (HBsAg) protein expression.
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KEYWORD
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perilla seedling, phenolic compounds, wounding, protein, protein expression
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